ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway

Figure 2

ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μm). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. versus the sham group; versus the db/db group; ^$ versus the db/db + I/R group; and ^£ versus the db/db + I/R + ZP2495 group.

(a)

(b)

(c)

(d)