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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 6581970, 11 pages
Research Article

Effect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin Resistance

1Department of Restorative Dentistry, Croydon University Hospital, 530 London Road, Croydon, Surrey CR7 7YE, UK
2Department of Physiology, Medical University of Bialystok, Mickiewicza 2c Str., 15-222 Bialystok, Poland
3County Veterinary Inspection, Zwycięstwa 26B Str., 15-959 Bialystok, Poland
4Department of Gastroenterology and Internal Medicine, Medical University of Bialystok, Sklodowskiej M.C. 24c Str., 15-274 Bialystok, Poland
5Department of Conservative Dentistry, Medical University of Bialystok, Sklodowskiej M.C. 24a Str., 15-274 Bialystok, Poland
6Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Mickiewicza 2c Str., 15-222 Bialystok, Poland

Correspondence should be addressed to Anna Zalewska; moc.liamg@624akswelaza

Received 15 August 2017; Accepted 3 December 2017; Published 30 January 2018

Academic Editor: Rodrigo Valenzuela

Copyright © 2018 Piotr Żukowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Oxidative stress plays a crucial role in the salivary gland dysfunction in insulin resistance (IR). It is not surprising that new substances are constantly being sought that will protect against the harmful effects of IR in the oral cavity environment. The purpose of this study was to evaluate the effect of N-acetylcysteine (NAC) on oxidative stress and secretory function of salivary glands in a rat model of insulin resistance. Rats were divided into 4 groups: C—normal diet, C + NAC—normal diet + NAC, HFD—high-fat diet, and HFD + NAC. We have demonstrated that NAC elevated enzymatic (superoxide dismutase, catalase, and peroxidase) and nonenzymatic antioxidants (reduced glutathione (GSH) and total antioxidant capacity (TAS)) in the parotid glands of HFD + NAC rats, while in the submandibular glands increased only GSH and TAS levels. NAC protects against oxidative damage only in the parotid glands and increased stimulated salivary secretion; however, it does not increase the protein secretion in the both salivary glands. Summarizing, NAC supplementation prevents the decrease of stimulated saliva secretion, seen in the HFD rats affected. NAC improves the antioxidative capacity of the both glands and protects against oxidative damage to the parotid glands of IR rats.