Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells

Figure 6

Effects of treatment with C. intybus, C. lanatus, and C. spinosum extracts at different concentrations for 24 h on ROS levels in EA.hy926 cells. The histograms of cell counts versus fluorescence of 10,000 cells analyzed by flow cytometry for the detection of ROS levels after treatment with (a) C. intybus, (b) C. lanatus, and (c) C. spinosum. FL2 represents the detection of fluorescence using 488 and 530 nm as the excitation and emission wavelength, respectively. Bar charts indicate the ROS levels as % of control as estimated by the histograms in EA.hy926 cells after treatment with C. intybus, C. lanatus, and C. spinosum extracts. All values of bar charts are presented as the mean ± SEM of 3 independent experiments. indicates significant difference from the control.

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