Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells

Table 1

The sequence of primers used for the assessment of mRNA levels of NFE2L2, GCLC, GSR, GPX1, HMOX1, CAT, NQO1, SOD1, and TXN genes in EA.hy926 cells by qRT-PCR.

Gene	Access no.	Primer (5-3)
CAT	847	Forward: CCAGAAGAAAGCGGTCAAGAA
		Reverse: TGGATGTGGCTCCCGTAGTC
SOD1	6647	Forward: AGGGCATCA TCAATTTCGAG
		Reverse: GGGCCTCAGACTACATCCAA
TXN	7295	Forward: TTTCCATCGGTCCTTACAGC
		Reverse: TTGGCTCCAGAAAATTCACC
HMOX1	3162	Forward: GGCCTGGCCTTCTTCACCTT
		Reverse: GAGGGGCTCTGGTCCTTGGT
NFE2L2	4780	Forward: ATTGCCTGTAAGTCCTGGTCA
		Reverse: ACTGCTCTTTGGACATCATTTCG
NQO1	1728	Forward: GGGCAAGTCCATCCCAACTG
		Reverse: GCAAGTCAGGGAAGCCTGGA
GCLC	2729	Forward: GAAGAAGATATTTTTCCTGTCATTGAT
		Reverse: CCATTCATGTATTGAAGAGTGAATTT
GSR	2936	Forward: CCAGCTTAGGAATAACCAGCGATGG
		Reverse: GTCTTTTTAACCTCCTTGACCTGGGAGAAC
GPX1	2876	Forward: CGCTTCCAGACCATTGACATC
		Reverse: CGAGGTGGTATTTTCTGTAAGATCA
GAPDH	2597	Forward: TGCACCACCAACTGCTTAG
		Reverse: GATGCAGGGATGATGTTC