Research Article
Oxidative Stress in Cardiac Tissue of Patients Undergoing Coronary Artery Bypass Graft Surgery: The Effects of Overweight and Obesity
Figure 2
Expression of regulatory proteins of the vascular tone in cardiac tissue. (a) Determination of the expression of endothelial NO synthase (eNOS) and its activated form, pSer1177-eNOS, by western blot analysis. The values are shown as percentage of control (normal BMI). Original blots are shown below the densitometric quantification. The signals of eNOS/pSer1177-eNOS were normalized to the loading control—beta-actin. (b) Expression of dihydrofolate reductase (DHFR) was measured by western blot analysis. Original blots are shown below the densitometric quantification. The signal of DHFR was normalized to the loading control—beta-actin. (c) The correlation between the BMI and the expression of DHFR is shown along the densitometric quantification (, ). Control: BMI < 25 kg/m2; overweight: BMI 25–29 kg/m2; obese: BMI > 30 kg/m2. All data are expressed as mean ± SEM from (a, b, c) independent measurements/patients. vs. Ctr. group; vs. Ctr. group.
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