Protective Effects of Miswak (Salvadora persica) against Experimentally Induced Gastric Ulcers in Rats

Table 2

Primer sequences.

Gene symbol	Gene description	GenBank accession number	Sequence	Annealing temperature (°C)
Actb	β-Actin	NM_031144.3	F: TGTTGTCCCTGTATGCCTCT; R: TAATGTCACGCACGATTTCC	60
eNOS	Endothelial nitric oxide synthase	NC_005103.4	F: TCTTCAAGGACCTACCTCAGGC; R: GCTAAGGCAAAGCTGCTAGGTC	60
TGF-β1	Transforming growth factor-β1	NM_021578.2	F: CCAACTACTGCTTCAGCTCCACA; R: TGTACTGTGTGTCCAGGCTCCAAA	58
TNF-α	Tumor necrosis factor-α	NM_012675.3	F: GACCCTCACACTCAGATCATCTTCT; R: TTGTCTTTGAGATCCATGCCATT	60
IL-1β	Interleukin-1β	NM_031512.2	F: CACCTCTCAAGCAGAGCACAG; R: GGGTTCCATGGTGAAGTCAAC	60