Epigallocatechin-3-Gallate (EGCG) Promotes Autophagy-Dependent Survival via Influencing the Balance of mTOR-AMPK Pathways upon Endoplasmic Reticulum Stress

Figure 5

EGCG pretreatment extends autophagy-dependent survival with respect to TM-induced ER stress. HEK293T cells were treated with 20 μM EGCG for 24 h followed by TM (25 μM) treatment for 2 h. (a) Meanwhile, the relative number of viable cells (upper panel) and relative cell viability (lower panel) were denoted in time. (b) The markers of autophagy (LC3, ULK-555-P), apoptosis (procaspase-3, PARP), AMPK (AMPK-P), and mTOR (4-EBP1-P), as well as ER stress markers (i.e., eiF2α-P and GADD34) were followed by immunoblotting in time. GAPDH was used as loading control. For each of the experiments, three independent measurements were carried out. Error bars represent standard deviation, and asterisks indicate statistically significant difference from the control: ; .

(a)

(b)