Defatted Kenaf (Hibiscus cannabinus L.) Seed Meal and Its Phenolic-Saponin-Rich Extract Protect Hypercholesterolemic Rats against Oxidative Stress and Systemic Inflammation via Transcriptional Modulation of Hepatic Antioxidant Genes

Table 2

Gene name, accession number, and primer sequences used in GeXP multiplex analysis.

Gene name	Accession number	Primer sequence (with universal tag)
		Forward	Reverse
Nrf2/Nfe2l2	NM_031789.2	AGGTGACACTATAGAATATCAGTTACAACTGGATGAAG	GTACGACTCACTATAGGGAGACTCATGGTCATCTACAAAT
Sod1	NM_017050	AGGTGACACTATAGAATAATATGGGGACAATACACAA	GTACGACTCACTATAGGGATCCAACATGCCTCTCT
Sod2	NM_017051	AGGTGACACTATAGAATACAGGTTGCTCTTCAGC	GTACGACTCACTATAGGGAAACTCTCCTTTGGGTTCT
Gsr	NM_053906.2	AGGTGACACTATAGAATAAATAAACTGGGGATTCAGAC	GTACGACTCACTATAGGGAAGTAGATTTTCACATTGTCTTTG
Gpx1	NM_030826	AGGTGACACTATAGAATATTGAGAAGTTCCTGGTAGGT	GTACGACTCACTATAGGGATTTTCTGGAAATCAGGTGT
B2m^a	NM_012512	AGGTGACACTATAGAATAATGCTTGCAGAGTTAAACA	GTACGACTCACTATAGGGATGCATAAAATATTTAAGGTAAGA
Kan(r)^c		GGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG	GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC
Hprt1^a,b	NM_012583	AGGTGACACTATAGAATATCCTCATGGACTGATTATG	GTACGACTCACTATAGGGACTGGTCATTACAGTAGCTCTT
Rpl13a^a	NM_173340	AGGTGACACTATAGAATAATGGGATCCCTCCAC	GTACGACTCACTATAGGGAATTTTCTTCTCCACATTCTT

^aHousekeeping genes; ^bnormalization gene; ^cinternal control supplied by Beckman Coulter Inc. (Brea, CA, USA).