Treatment of NASH with Antioxidant Therapy: Beneficial Effect of Red Cabbage on Type 2 Diabetic Rats

Table 2

Oxidative stress levels and rat hepatic functions at 4 months after the administration of different diets.

Variables	ND	HFHF	HFHF/ND	HFHF/NDRC	HFHF/HFHFRC
Plasma oxidative stress parameters
Plasma total antioxidant capacity (mM trolox equivalent)	5.75 ± 0.18	5.75 ± 1.167	5.99 ± 0.131	6.43 ± 0.35^$	6.18 ± 0.26
Plasma lipid peroxide, TBARS; (μM malondialdehyde)	35 ± 1.14	66.98 ± 9.57	34.7 ± 0.925^$$	34.67 ± 3.097^$$	41.93 ± 6.02^$
Catalase activity (μmol/L); SOD activity (% inhibition)	0.0516 ± 0.03; 57.52 ± 5.83	0.022 ± 0.004; 71.43 ± 4.22	0.039 ± 0.011; 81.11 ± 1.98	0.044 ± 0.010$; 80.9 ± 1.36	0.043 ± 0.006; 81.11 ± 2.49
Vascular oxidative stress
DHE fluorescence (% vs. ND)	100 ± 18.5	183.3 ± 7.1	160.9 ± 17.1	124.2 ± 7.4$$	200.2 ± 17.1
eNOS fluorescence (% vs. ND)	100 ± 10.7	53.3 ± 7.2	78 ± 3.01^$	131.5 ± 10^$$$	111.05 ± 18.05^$
Nitrotyrosine fluorescence (% vs. ND)	100 ± 8.3	113.5 ± 15	107.4 ± 7.8	100.3 ± 11.2	162.1 ± 31.3
Hepatic oxidative stress
Catalase activity (g/mL); SOD activity (% inhibition)	0.50 ± 0.01; 99.9 ± 0.39	0.49 ± 0.01; 105.4 ± 5.09	0.53 ± 0.012^$$; 103.3 ± 1.84	0.53 ± 0.006^$$; 99.75 ± 1.33	0.47 ± 0.003; 103.3 ± 0.78
Hepatic function
Liver weight (g); (% vs. rat weight)	17.12 ± 0.45; 3.07 ± 0.03	22.28 ± 2.01; 3.50 ± 0.28	17.83 ± 0.78^$; 3.13 ± 0.12	17.53 ± 1.12^$; 3.28 ± 0.13	21.37 ± 1.25; 3.71 ± 0.16^#
Triglycerides (nmol/mg of liver)	1.8 ± 0.1	19.9 ± 2.4	2.7 ± 0.3^$$$	2.2 ± 0.3^$$$	6.1 ± 1.1^$$$
Cholesterol (mg/mg of liver)	7.8 ± 0.7	32.7 ± 9.9	10.6 ± 1.6^$$	13.9 ± 1.6^$	14.2 ± 2.5^$
Glycogen storage (mg/mg of liver)	0.0417 ± 0.0034	0.02405 ± 0.0027	0.04615 ± 0.0071^$	0.0335 ± 0.001	0.0446 ± 0.0084^$
Plasma γGT (U/L)	17.3 ± 0.58	34.1 ± 11.08	25.9 ± 0.35	27.2 ± 0.69	28.78 ± 2.85