MicroRNA miR-24-3p Reduces Apoptosis and Regulates Keap1-Nrf2 Pathway in Mouse Cardiomyocytes Responding to Ischemia/Reperfusion Injury

Figure 4

(a) The putative binding site of miR-24-3p is located in the 3-UTR of Keap1 mRNA. (b) The double luciferase reporter assay system is used to detect the direct interaction of miR-24-3p with the Keap1 3-UTR. Data show the comparison of the relative luciferase activity of the vector harboring wild-type or mutant Keap1 3-UTR following the transfection of miR-24-3p mimic (green bars) or nontransfection control (miR-NC) (black bars). .

(a)

(b)