Isoliquiritigenin Ameliorates Acute Pancreatitis in Mice via Inhibition of Oxidative Stress and Modulation of the Nrf2/HO-1 Pathway

Figure 3

ISL reduced the oxidative stress response of pancreatic tissue in mice with MAP. (a) A representative immunofluorescence image of DHE. ROS activity was measured by fluorescent-labeled DHE staining. Stained sections of pancreas in magnification 200x. (b–d) Protein levels of nuclear Nrf2 and HO-1 in the pancreatic tissues were analyzed by Western blotting. Lamin B1 and β-actin were used as a control for protein loading. (e) Levels of oxidative stress products (SOD, MDA, and GSH) of pancreas. , , and versus V/Cae group. each group. V represents vehicle; S represents saline; L, M, and H represent low-dose (50 mg/kg), medium-dose (100 mg/kg), and high-dose ISL (200 mg/kg). R represents 100 mg/kg resveratrol.

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