Acute Hyperbaric Oxygenation, Contrary to Intermittent Hyperbaric Oxygenation, Adversely Affects Vasorelaxation in Healthy Sprague-Dawley Rats due to Increased Oxidative Stress

Figure 8

(a) DHE superoxide fluorescence intensity measurement in the rat femoral artery. The increase in superoxide levels in the artery of rats exposed to a single session of hyperbaric oxygen was prevented by incubation with TEMPOL. Data are shown as of DHE fluorescence intensity units. Significant increase in superoxide levels compared with control value before any treatment (; rats). (b) In situ fluorescence of superoxide after dying of the femoral artery with DHE color in all tested groups before and after incubation with TEMPOL. The color intensity is proportional to the amount of superoxide. The images were made on the Zeiss Axioskop MOT2 microscope, using the Olympus DP70 camera with the Zeiss filter set 15 (546 nm wavelength for excitation and 590 nm for emission with a beam splitter at 580 nm).

(a)

(b)