Research Article
Increased F2-Isoprostane Levels in Semen and Immunolocalization of the 8-Iso Prostaglandin F2α in Spermatozoa from Infertile Patients with Varicocele
Figure 1
Immunocytochemical staining with polyclonal 8-iso-PGF2α antibody of sperm from fertile man (a), idiopathic infertile man (b), infertile patients with varicocele (c, d). In the sperm from a fertile man, the signal is absent; in the sperm from an idiopathic infertile man, the label is dotted (arrow) and located in the midpiece of the sperm tail. A high percentage of spermatozoa from infertile patients with varicocele shows an intense staining in the cytoplasmic residues surrounding the head and the tail and in the midpiece (arrows). Bar: 5 μm.
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