Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat

Figure 1

Surface markers of EPC and MSC and fluorescent intensity of mitotracker staining in H9C2 cells undergoing menadione and SVF treatments. (a–n) Flow cytometry for endothelial progenitor cell (EPC), cardiac stem cell, and mesenchymal stem cell (MSC) surface markers. (o) Flow cytometric analysis () showed that CD34 and CD271 were the clearest EPC and MSC surface markers for SVF, respectively. (p, 1–8) Illustrating the immunofluorescent (IF) microscopic finding of the mitotracker stain for identification of mitochondria in H9C2 cells in the normal control group (p, 1 and 2) and AD-SVF treated group (p, 3 and 4). The number of mitotracker emission positive cells (i.e., counted in five randomly chosen fields) was significantly increased after receiving SVF treatment, vs. †, (red symbols). (p, 5–8) Illustrating the IF microscopic finding of the mitotracker stain for identification of mitochondria in H9C2 cells in the menadione-treated group (p, 5 and 6) and menadione + AD-SVF treated group (p, 7 and 8). The number of mitotracker emission positive cells was significantly increased in menadione + SVF treatment than in menadione only, ‡ vs. §, (green symbols). Blue color indicated nuclei stained by DAPI. in each group. AD-SVF = adipose-derived stromal vascular fraction.

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