Research Article
Oxygen Concentration and Oxidative Stress Modulate the Influence of Alzheimer’s Disease Aβ1–42 Peptide on Human Cells
Figure 10
Percentage of apoptotic and necrotic cells after treatment with Aβ1–42 peptide and/or X-ray irradiation. (a) In cells cultivated at 21% O2, percentage of apoptotic and necrotic cells was only slightly increased (~1.1- or 1.2-fold) 24 h after Aβ peptide treatment or 18 h after irradiation (2 Gy), whereas combination of both resulted in a slight decrease in cell death (apoptosis and necrosis) compared to control cells. (b) In cells at 5% O2, Aβ peptide treatment or irradiation led to a significant (~1.3- or 1.5-fold) increase in percentage of dead cells. The combination of both amyloid beta treatment and irradiation resulted in the decrease (up to 1.3-fold) of cell death below its level in control sample. Samples were analyzed at least in duplicates (–6) in three independent experiments (), and data is analyzed by two-way ANOVA, followed by Tukey’s posttests. ( and ).
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