Oxygen Concentration and Oxidative Stress Modulate the Influence of Alzheimer’s Disease Aβ_1–42 Peptide on Human Cells

Figure 7

Level of protein carbonylation. Cells cultivated at 21% O₂ showed severely increased (about 1.8- to 2.5-fold) protein carbonylation compared to cells at 5% O₂, and this increase at 21% O₂ only was significantly pronounced 18 h after irradiation. Aβ peptide treatment for 24 h alone or combined with irradiation resulted in an increase (~1.6- and 1.4-fold, resp., not significant) in protein carbonylation in cells at 5% O₂, whereas only a minor increase (1.15-fold) after Aβ peptide only was observed at 21% O₂. Combination of two stressors did not lead a further increase in protein carbonylation at 21% O₂. Samples were measured at least in three independent experiments. Mean ± SEM analyzed by two-way ANOVA with Dunnet’s multiple comparison test. (, , and ).