Oxygen Concentration and Oxidative Stress Modulate the Influence of Alzheimer’s Disease Aβ_1–42 Peptide on Human Cells

Figure 8

Mitochondrial DNA amount upon Aβ_1–42 treatment and/or irradiation in SH-SY5Y cells. (a, b) Aβ peptide or irradiation (2 Gy X-rays) alone affected mtDNA amount depending on O₂ level in the cell culture: about 1.2- and 1.3-fold, respectively, decrease at 21% O₂ and up to 1.3- and 1.5-fold (a), respectively, increase at 5% O₂ (b) compared to respective controls. The combined effect of Aβ peptide and irradiation led to a decrease in mtDNA amount at both oxygen concentrations, particularly at 21% O₂ (1.8-fold) after 1 day/18 h with almost no change observed after 3 days/54 h, whereas at 5% O₂ it was restored to the level of control sample. Mean ± SEM analyzed by two-way ANOVA with Dunnet’s multiple comparison test. (, , and ).

(a)

(b)