Polyphenol Stilbenes from Fenugreek (Trigonella foenum-graecum L.) Seeds Improve Insulin Sensitivity and Mitochondrial Function in 3T3-L1 Adipocytes
Effects of polyphenol stilbenes on viability and differentiation of 3T3-L1 adipocytes. (a) 3T3-L1 preadipocytes were incubated with different concentrations of polyphenol stilbenes for 48 hr after which cell viability was determined by extracellular LDH assay in the media. Data are expressed as the mean ± SD of three independent experiments. (b) 3T3-L1 preadipocytes were induced to full differentiation for 8 days as described in Figure 2. Lipid droplets were stained by Oil Red O. Con: undifferentiated 3T3-L1 preadipocytes; Mod: fully differentiated 3T3-L1 adipocytes. (c) On day 8, the cellular triglyceride (TG) content was measured and normalized against total protein. Data are presented as the mean ± SD of three independent experiments (). mature adipocytes versus preadipocytes; , compound-treated adipocytes versus mature adipocytes. (d) Adipocyte-specific protein expression on day 8 was determined by Western blot analysis.
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