Myoblast Myogenic Differentiation but Not Fusion Process Is Inhibited via MyoD Tetraplex Interaction

Figure 2

Immunofluorescence against MHC (green) on differentiating C2C12 cell culture untreated or treated with 12 mM of TMPyP4. (a–d) Untreated myoblast. In (a) 10x and (c) 20x, nuclei were counterstained by DAPI (blue). In (b) 10x and (d) 20x, immunofluorescence was superimposed on phase-contrast image. (e–h) TMPyP4-exposed differentiating C2C12 cells. In (e) 10x and (g) 20x, immunofluorescence was superimposed on phase-contrast image and nuclei were labelled by DAPI (blue). In (f) 10x and (h) 20x, no MHC presence was detected. Elliptical shapes highlighted myotube and elongated structure resembling a myotube not expressing MHC upon C2C12 cell TMPyP4 exposure.

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