Myoblast Myogenic Differentiation but Not Fusion Process Is Inhibited via MyoD Tetraplex Interaction

Figure 4

Western blot analysis for Myog and MHC protein expressions in differentiating C2C12-treated and untreated cells. (a) Representative Western blot filters labelled with antibodies against Myog, MHC, and vinculin as the loading control. (b) Optical densitometry analysis for quantitative evaluation of Myog and MHC expression normalized with vinculin. The differences between treated cell (grey bars) and control cells (black bars) are strongly significant both for MyoG and MHC protein expression (; ).

(a)

(b)