Andrographolide Ameliorates Liver Fibrosis in Mice: Involvement of TLR4/NF-κB and TGF-β1/Smad2 Signaling Pathways

Figure 5

Andro suppressed proinflammatory chemokines mediated by TLR4/NF-κB p50 signaling in HSC. (a–c) The mRNA levels of IL-1β, IL-6, and MCP-1 were measured by q-PCR. (d) The protein expression of TLR4, p-p50, and p50 was examined by Western blot. (e, f) p50 levels in the cytosol and nucleus were assayed. (g) Representative immunofluorescent staining of TLR4. (h) Nuclear translocation of NF-κB p50 in HSC was assayed by immunofluorescence. . , , and versus control.

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