Research Article
The Endothelin Receptor Antagonist Macitentan Improves Isosorbide-5-Mononitrate (ISMN) and Isosorbide Dinitrate (ISDN) Induced Endothelial Dysfunction, Oxidative Stress, and Vascular Inflammation
Figure 9
Characterization of vascular mRNA expression within the inflammatory and ET receptor signaling pathways and protective effects of macitentan cotherapy in ISDN-treated mice. qRT-PCR was used to determine mRNA expression levels of Nox-2 (a), ECE-1 (b), MCP-1 (c), and IL-6 (d). Immunohistochemical staining for ET-1 in aortic paraffin sections (e). Arrows indicate ET-1-specific brown color. Representative images for 4 independent experiments. The data are mean ± SEM from 5 (a), 8-9 (b), 3 (c), and 3 (d) mice per group. vs. control; vs. ISDN group.
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