Anti-Inflammatory Effect of a Polyphenol-Enriched Fraction from Acalypha wilkesiana on Lipopolysaccharide-Stimulated RAW 264.7 Macrophages and Acetaminophen-Induced Liver Injury in Mice

Figure 10

Effect of PEF on the activation of TLRs, MAPKs, and NF-κB in APAP-intoxicated mouse livers. (a) Total protein was extracted from liver tissues, and the protein levels of TLR3, TLR4, p-JNK, JNK, p-ERK, ERK, p-p38, p-38, p-IKKα/β, IKKα/β, p-IκB-α, IκB-α, p-NF-κB, and NF-κB were determined by Western blot analysis. GAPDH was used as an endogenous control. (b) Total RNA was isolated from liver tissues and reverse-transcribed into cDNA for RT-PCR analysis of TLR3 and TLR4 mRNA level. GAPDH was used as an endogenous control. Results are shown as the mean ± SD (). The different letters represent the statistical differences at among the groups by Tukey-Kramer’s test.

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