Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells

Figure 4

Effects of cafestol on HO-1 expression in the presence of cyclic strain treatment. HUVECs were treated with cafestol 12 h prior to cyclic strain treatment for 12 h. (a) The mRNA level of HO-1 was analyzed through qPCR and normalized to GAPDH. (b) Immunoblotting of HO-1 and GAPDH was performed, and the bands were quantitated using ImageJ. The data represent the of three independent experiments. versus untreated control; versus cells exposed to cyclic strain. (c) Effects of the inhibition of HO-1 on protein expression levels as examined using ELISA. HUVECs were pretreated for 30 min with Znpp, and cafestol (10 μM) was then added 12 h prior to cyclic strain treatment for 24 h. Data represent the of four independent experiments. versus untreated controls; versus cells exposed to cyclic strain. versus cells exposed to cyclic strain plus cafestol treatment.

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