Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells

Figure 5

Effects of cafestol and the Sirt1 inhibitor sirtinol on cyclic-strain-induced inflammatory molecule protein expression in HUVECs. (a) Effects of cafestol on Sirt1 expression. Cells were pretreated with cafestol for 12 h or sirtinol for 2 h and then exposed to cyclic strain for 24 h. Protein expression was examined using Western blotting. The data are representative of three independent experiments. The bar graph shows the fold increase in protein expression compared with control cells. (b) Effects of the inhibition of Sirt1 by sirtinol on protein expression levels as examined using ELISA. HUVECs were pretreated with cafestol for 12 h or sirtinol for 2 h prior to cyclic strain treatment for 24 h. (c) Effects of Sirt1 siRNA on protein expression levels as examined using ELISA. Transfected cells were pretreated with 10 μM cafestol for 12 h, then subjected to cyclic strain treatment for 24 h. Data represent the of four independent experiments. versus untreated controls; versus cells exposed to cyclic strain. versus cells exposed to cyclic strain plus cafestol treatment.

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