Flutamide Induces Hepatic Cell Death and Mitochondrial Dysfunction via Inhibition of Nrf2-Mediated Heme Oxygenase-1

Figure 6

Effects of HO-1 interventions on flutamide-induced oxidative stress and mitochondrial dysfunction. Cells were pretreated with or without 20 μM Snpp/10 μM Copp for 1 h followed by treatment with 50 μM flutamide. After completion of drug treatments, the levels of hydrogen peroxide (a), mitochondrial membrane potential (b), and ATP (c) as well as the protein expressions of Nrf2, HO-1, and SOD2 were analyzed (d–f). Data are presented as the mean ± SD from 3 independent experiments. compared with the cells in the control group without drug treatment; compared with the cells treated with flutamide.

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