Oleuropein Aglycone Protects against MAO-A-Induced Autophagy Impairment and Cardiomyocyte Death through Activation of TFEB

Figure 1

OA induces autophagy in neonatal rat cardiomyocytes. (a) Rat neonatal ventricular myocytes were treated with OA (100 μM) for 1 h, and autophagic vacuoles were stained with Cyto-ID green (left panel). Nuclei were counterstained with Hoechst 33342. Scale bar 20 μm. The graph on the right panel represents quantification of fluorescence intensity ( independent experiments; for each experiment, 6 fields with about 15 cells were quantified per condition). (b, c) Immunoblot analysis of Beclin1 and LC3 proteins was performed on cardiomyocyte protein extracts after stimulation with OA (100 μM) for 1 h. β-Actin expression was used as loading control. The graphs represent quantifications of Beclin1/β-actin () and LC3-II/LC3-I ratios () measured by densitometry analysis. , , and versus CTRL (control).

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