Oleuropein Aglycone Protects against MAO-A-Induced Autophagy Impairment and Cardiomyocyte Death through Activation of TFEB

Figure 2

OA enhances autophagic flux in neonatal rat cardiomyocytes. (a) Double immunofluorescence imaging of RFP-GFP-LC3 in cardiomyocytes stimulated with 100 μM OA for different times (1 h, 3 h, and 6 h) (left panel). Scale bar 20 μm. Quantification of yellow puncta (autophagosomes) and red puncta (autolysosomes) for each condition is displayed on the histogram (right panel) ( independent experiments; for each experiment, 10 cells were quantified per condition). versus CTRL; versus CTRL; OA 3 h versus 6 h; OA 3 h versus 6 h. (b) Immunoblot analysis of LC3 protein was performed on cardiomyocyte protein extracts after stimulation with chloroquine (CLQ, 10 μM) or CLQ + OA (CLQ, 10 μM, and OA, 100 μM) for 6 h. β-Actin expression was used as loading control. The graphs represent quantifications of the LC3-II/LC3-I ratio measured by densitometry analysis (). versus CTRL; versus CTRL; CQ versus CQ + OA.

(a)

(b)