Oleuropein Aglycone Protects against MAO-A-Induced Autophagy Impairment and Cardiomyocyte Death through Activation of TFEB

Figure 3

OA induces TFEB translocation and activation. (a) Immunofluorescence staining with TFEB antibody in cardiomyocytes treated with OA (100 μM) for 30 min (red). Nuclei were counterstained with DAPI. Scale bar 20 μm (left side). Quantification analysis of cells with nuclear localization of TFEB in percent of total nuclei (right side) ( independent experiments; for each experiment, 6 fields with about 15 cells were quantified per condition). (b) Real-time PCR expression of Atp6v1, p62, and Lamp1 normalized to GAPDH transcript levels in cardiomyocytes treated with OA (100 μM) (). versus CTRL.

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