Oleuropein Aglycone Protects against MAO-A-Induced Autophagy Impairment and Cardiomyocyte Death through Activation of TFEB

Figure 8

The protective effects of OA on MAO-A-induced toxicity are lost upon TFEB silencing. (a) Cardiomyocytes were silenced with scramble (SCR) or TFEB siRNA, and the level of TFEB mRNA was examined 48 h posttransfection by real time-RT-PCR. Results are normalized to GAPDH and expressed as a fold increase to SCR control siRNA (). (b) Cardiomyocytes were transfected with SCR or TFEB-siRNA for 24 h and then transduced with MAO-A adenovirus for an additional 24 h. Then, cardiomyocytes were stimulated with TYR (500 μM) for 6 h or TYR + OA in the last 4 h, and an MTT test was performed (). and .

(a)

(b)