Genetically Engineered Resveratrol-Enriched Rice Inhibits Neuroinflammation in Lipopolysaccharide-Activated BV2 Microglia Via Downregulating Mitogen-Activated Protein Kinase-Nuclear Factor Kappa B Signaling Pathway

Figure 4

Treatment with resveratrol-enriched rice modulates MAPK signaling in lipopolysaccharide-activated (acute) BV2 microglial cells. BV2 microglial cells were activated with LPS (100 ng/mL) 30 min before sample treatment, and samples of different concentrations were added under the same condition. Incubation of LPS and sample was performed for 30 min. MAPK expression was measured in pretreatment in LPS-activated BV2 cells. (a, b) JNK/pJNK expression and band intensity. (c, d) ERK/pERK expression and band intensity. (e, f) p38/pP38 expression and band intensity in LPS-activated BV2 microglia. All data are presented as the mean ± standard error of the mean of three independent experiments. , , and indicate significant differences compared with LPS treatment alone. indicates significant differences compared with untreated control group. indicates significant differences to RR compared to NR. Ctl, untreated control and LPS, lipopolysaccharide.

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