Gefitinib Inhibits Bleomycin-Induced Pulmonary Fibrosis via Alleviating the Oxidative Damage in Mice

Figure 7

Gefitinib treatment decreased gene and protein expressions of NOX1/2/4 in the lung tissues affected by pulmonary fibrosis. Lung tissues from gefitinib- (Ge-) treated and PBS-treated pulmonary fibrosis mice were collected two weeks after bleomycin (BLM) administration. (a) The NOX1/2/4 gene expressions were identified by reverse transcriptase polymerase chain reaction (RT-PCR). (b) The relative mRNA folds were calculated. (c) The NOX1/2/4 protein expressions were identified by Western blot. (d) The relative band densities were calculated. The NOX1/2/4 gene and protein expressions in the lung tissues were significantly increased after BLM administration. However, gefitinib treatment significantly inhibited NOX1/2/4 expressions. All data were expressed as the mean ± SD, . versus BLM group.

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