Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells

Figure 2

2.6 and 18 nm AgNPs induced RNS generation and changes in NOS protein and mRNA level in PANC-1 cells. (a) NO. (b) NO₂, changes in NOS protein level: (c) representative Western blot analysis of nNOS, iNOS, and eNOS. (d) nNOS, (e) iNOS, and (f) eNOS. mRNA level of (g) nNOS, (h) iNOS, and (i) eNOS in PANC-1 cells after treatment for 24. Control (+): cells treated with LPS for 24 h; control: untreated cells. Data are expressed as mean ± SD of 4 independent experiments. ; exposed cells versus control.

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