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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 8267560, 10 pages
Research Article

Noninvasive Real-Time Characterization of Renal Clearance Kinetics in Diabetic Mice after Receiving Danshensu Treatment

1Department of Health Technology and Informatics, Hong Kong Polytechnic University, Kowloon, Hong Kong
2School of Biomedical Sciences, Chinese University of Hong Kong, New Territories, Hong Kong
3School of Medical Science and Menzies Health Institute Queensland, Griffith University, Gold Coast, Australia

Correspondence should be addressed to Andrew C. Bulmer; ua.ude.htiffirg@remlub.a and Christopher W. K. Lai; kh.ude.uylop@ial.sirhc

Received 17 August 2017; Revised 28 November 2017; Accepted 31 December 2017; Published 12 February 2018

Academic Editor: Evangelia Dounousi

Copyright © 2018 Lei Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Danshensu (DSS) is an active ingredient extracted from the root of the Danshen that could ameliorate oxidative stress via upregulation of heme oxygenase- (HO-) 1. Little is known about the treatment effects of DSS on kidney function in diabetic mice. Therefore, the primary aim of the present study was to characterize the renal clearance kinetics of IRdye800CW in db/db mice after DSS treatment. The secondary aim was to measure several biomarkers of renal function and oxidative stress (urinary F2-isoprostane, HO-1 in kidney and serum bilirubin). Fourteen db/db diabetic mice were randomly assigned into two groups and received either DSS treatment (DM + DSS) or vehicle treatment (DM). A third group that comprised of db/+ nondiabetic mice (non-DM control) received no DSS treatment and served as the nondiabetic control. At the end of a 3-week intervention period, serum and urinary biomarkers of renal function and oxidative stress were assessed and the renal clearance of IRdye800CW dye in all mice was determined noninvasively using Multispectral Optoacoustic Tomography. The major finding from this study suggested that DSS treatment in db/db mice improved renal clearance. Increased expression of HO-1 after DSS treatment also suggested that DSS might represent a potential therapeutic avenue for clinical intervention in diabetic nephropathy.