Protective Effects of Taraxasterol against Ethanol-Induced Liver Injury by Regulating CYP2E1/Nrf2/HO-1 and NF-κB Signaling Pathways in Mice
Effects of taraxasterol on the expression of CYP2E1 in liver tissues of mice with ethanol-induced liver injury. The mice were treated with taraxasterol (2.5, 5, and 10 mg/kg, respectively) or TPN and induced with EtOH as described in Materials and Methods. (a) The expression of CYP2E1 in liver tissues was examined by immunohistochemical analysis (200x, brown yellow granules indicate positive reaction). (A) Normal group; (B) EtOH group; (C) TPN group; (D) 10 mg/kg of taraxasterol group; (E) 5 mg/kg of taraxasterol group; (F) 2.5 mg/kg of taraxasterol group. (b) The expression of CYP2E1 in liver tissues was examined by western blot analysis. The relative expression of CYP2E1 protein was normalized to β-actin. The values represent the means ± SEMs. vs. normal group; and vs. EtOH group.