Nrf2 Deficiency Unmasks the Significance of Nitric Oxide Synthase Activity for Cardioprotection

Table 1

Concentrations (nM) of low-molecular-weight thiols in blood and tissues of wild type (WT) and Nrf2 KO mice (mean ± SD, per group).

	WT mice	Nrf2 KO mice	value (versus WT mice)
Heart
Total GSH (nM)	144.3 ± 53.11	91.52 ± 12.06	=0.0620 (↓ ns)
GSH (nM)	143.7 ± 52.77	90.77 ± 11.99	=0.0602 (↓ ns)
GSSG (nM)	0.6263 ± 0.427	0.7531 ± 0.352	0.6225
GSH/GSSG	335.0 ± 217.9	140.2 ± 52.51	=0.0887 (↓ ns)
Cys (nM)	16.38 ± 6.976	8.99 ± 1.804	=0.051 (↓ ns)
HCys (nM)	0.6387 ± 0.1949	0.4231 ± 0.06714	↓0.0475
GluCys (nM)	0.2066 ± 0.03208	0.1852 ± 0.02159	0.2506
Sulfide (nM)	45.9 ± 22.1	28.56 ± 6.597	0.1301
Aorta
Total GSH (nM)	217.2 ± 73.95	105.9 ± 21.91	↓0.0121
GSH (nM)	216.4 ± 73.54	105.2 ± 22.2	↓0.0119
GSSG (nM)	1.099 ± 0.2598	0.7146 ± 0.4136	0.1517
GSH/GSSG	224.8 ± 58.4	186.9 ± 101.2	0.5308
Cys (nM)	54.18 ± 12.78	18.15 ± 3.143	↓0.0003
HCys (nM)	2.324 ± 0.3268	3.226 ± 2.024	0.3539
GluCys (nM)	0.9574 ± 0.1772	0.6319 ± 0.1166	↓0.0089
Sulfide (nM)	21.51 ± 5.773	16.78 ± 5.570	0.2237
Liver
Total GSH (nM)	8097 ± 833.6	4742 ± 2042	↓0.0094
GSH (nM)	8028 ± 814.8	4592 ± 2025	↓0.0078
GSSG (nM)	68.61 ± 27.77	150.6 ± 43.52	↑0.0075
GSH/GSSG	127.2 ± 31.83	31.55 ± 15.14	↓0.0003
Cys (nM)	204.8 ± 16.85	179.1 ± 52.44	0.3272
HCys (nM)	39.12 ± 7.435	18.18 ± 6.563	↓0.0015
GluCys (nM)	0.7695 ± 0.04889	0.5463 ± 0.1343	↓0.0082
CysGly (nM)	96.75 ± 16.4	62.13 ± 21.8	↓0.0219
Sulfide (nM)	13.19 ± 2.683	15.17 ± 4.521	0.4229
Plasma
Total GSH (nM)	27,144 ± 8464	16,594 ± 3540	↓0.0331
GSH (nM)	26,456 ± 8364	16,194 ± 3482	↓0.0351
GSSG (nM)	688.3 ± 171	399.9 ± 93.14	↓0.0107
GSH/GSSG	39.04 ± 10.17	41.28 ± 7.372	0.7005
Cys (nM)	11,900 ± 2432	8207 ± 1249	↓0.0165
HCys (nM)	337 ± 159	209.2 ± 35.86	0.1177
GluCys (nM)	668.8 ± 140.9	320.1 ± 83.34	↓0.0014
CysGly (nM)	725 ± 186	1422 ± 728.2	= 0.0751 (↓ ns)
Sulfide	332.1 ± 46.77	457.7 ± 386.6	0.4912
Erythrocytes
Total GSH (nM)	3.9410^6 ± 0.5210^6	3.7710^6 ± 0.3010^6	0.5570
GSH (nM)	3.9310^6 ± 0.5210^6	3.7710^6 ± 0.3010^6	0.5583
GSSG (nM)	1058 ± 103.7	781.5 ± 153.3	↓0.0103
GSH/GSSG	3766 ± 726.2	4946 ± 841	↑0.0449
Cys (nM)	26,254 ± 10,655	18,796 ± 2980	0.1702
HCys (nM)	754.3 ± 317.9	550.8 ± 104.8	0.2109
GluCys (nM)	286.7 ± 118.3	202.7 ± 44.78	0.1757
CysGly (nM)	11,488 ± 5053	11,772 ± 3183	0.9178
Sulfide (nM)	387.3 ± 69.59	490.7 ± 98.66	0.0917

ns - not significant; ↓ - decreased; ↑ - increased; , , .