Protective Effect of Rosamultin against H₂O₂-Induced Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes

Figure 11

The effect of rosamultin on the PI3/Akt signaling pathway in H₂O₂-treated H9c2 cells. Cells were precultured in the presence or absence of 40 μM LY294002, a specific inhibitor of PI3K, and incubated with or without rosamultin for 24 h; after that, cells were stimulated further with 200 μM H₂O₂ for an additional 3 h. (a) Effect of rosamultin on H₂O₂-induced cell damage in the presence and absence of a PI3K inhibitor. Cell vitality was measured by MTT assay. (b) Representative Western blots. (c) pAkt protein expression. (d) Caspase-3 protein expression. Data are mean ± S.E.M. (). versus the control group, versus the model group, versus the model group, and versus the rosamultin pretreatment group.

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