miR-26a Potentially Contributes to the Regulation of Fatty Acid and Sterol Metabolism In Vitro Human HepG2 Cell Model of Nonalcoholic Fatty Liver Disease

Table 1

List of primers and TaqMan probes used.

(a)

Gene	Forward primer (5-3)	Reverse primer (5-3)
GAPDH	GAAGGTGAAGGTCGGAGTCAA	CAGAGTTAAAAGCAGCCCTGGT
SREBP1c	CAGCCCACTTCATCAAGG	ACTGTTGCCAAGATGGTTCCG
FASN	AACTCCTGCAAGTTCTCCGA	GCTCCAGCCTCGCTCTC
SCD1	GACGATGAGCTCCTGCTGTT	CTCTGCTACACTTGGGAGCC
CD36	GGCTGTGACCGGAACTGTG	AGGTCTCCAACTGGCATTAGAA
DGAT1	TATTGCGGCCAATGTCTTTGC	CACTGGAGTGATAGACTCAACCA
PLIN2	ATGGCATCCGTTGCAGTTGAT	GGACATGAGGTCATACGTGGAG
PLIN4	GGAGCTGCAACCTTCGGAAA	GGACCACTCCCTTAGCCAC
ApoB	AGAGGACAGAGCCTTGGTGGAT	CTGGACAAGGTCATACTCTGCC
IL-6	GTGGAGATTGTTGCCATCAACG	CAGTGGATGCAGGGATGATGTTCT
TGFβ1	CCCAGCATCTGCAAAGCTC	GTCAATGTACAGCTGCCGCA
TGFβ2	CAGCACACTCGATATGGACCA	CCTCGGGCTCAGGATAGTCT
hATF6	AGCAGCACCCAAGACTCAAAC	GCATAAGCGTTGGTACTGTCTGA
hCHOP	AGGAACCAGGAAACGGAAACAGA	TCTCCTTCATGCGCTGCTT
hIRE1	GCCGAAGTTCAGATGGAATC	ATCTGCAAAGGCCGATGA
BECN1	GGTGTCTCTCGCAGATTCATC	TCAGTCTTCGGCTGAGGTTCT
LC3	GATGTCCGACTTATTCGAGAGC	TTGAGCTGTAAGCGCCTTCTA
POLR3G	GAGGACGTGCTGCTTATACCT	CTGTTCTGCGGCATCATCGT
TAB3	TGTACTCCATCACCATCTCCT	TGCTTTGCTAACCTCTCCAT
PKCδ	AAAGGCAGCTTCGGGAAG	TGGATGTGGTACATCAGGTC

(b)

TaqMan miRNA assay (5-3)
hsa-miR-26a-5p	UUCAAGUAAUCCAGGAUAGGC
U6 snRNA	GTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAGATTAGCAT GGCCCCTGCGCAAGGATGACACGCAAATTCGTGAAGCGTTCCATATTTT