Inhibition of Protein Aggregation by Several Antioxidants

Figure 2

Aggregation kinetics of stefin B monitored by ThT binding/fluorescence in the absence and presence of different concentrations of NAC. ThT fluorescence emission at 482 nm was monitored upon excitation at 440 nm. Protein concentration was 34 μM, and concentrations of NAC were varied. Each sample was incubated at room temperature in 0.015 M acetate buffer, 0.15 M NaCl at pH 4.8, prior to mixing with the ThT probe as described in Section 2.