Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 3

Research Article

6-O-Galloylpaeoniflorin Attenuates Cerebral Ischemia Reperfusion-Induced Neuroinflammation and Oxidative Stress via PI3K/Akt/Nrf2 Activation

Figure 3

GPF upregulates and activates Nrf2. PC12 cells were treated with different doses of GPF (0, 10, 50, or 100 μM, resp.) for 6 h or treated with PI3K/Akt inhibitor Ly294002 (50 μM) for 1 h followed by treatment with GPF (100 μM) for 6 h. The results of Western blot analysis showed that Nrf2 and p-Akt expression levels were upregulated by 10–100 μM GPF, which were inhibited by pretreatment with the PI3K/Akt inhibitor Ly294002. GPF treatment has no effect on Akt expression in PC12 cells (a). Fluorescence staining showed that 10–100 μM GPF treatment induced Nrf2 (red fluorescence) translocation into the nuclei (blue). Pretreatment with Ly294002 significantly attenuated the GPF-induced nuclear translocation of Nrf2 (b). The data of each group were from three independent assays. versus the control group; versus the control group; versus the 100 μM GPF group.
(a)
(b)

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