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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 8713263, 16 pages
https://doi.org/10.1155/2018/8713263
Research Article

Citrus aurantium Naringenin Prevents Osteosarcoma Progression and Recurrence in the Patients Who Underwent Osteosarcoma Surgery by Improving Antioxidant Capability

1Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun 130033, China
2Department of Nephrology, China-Japan Union Hospital of Jilin University, Changchun 130033, China
3Cardiology Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China
4Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China
5Teaching Office, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
6Pediatrics, Liuhe District Hospital of Nanjing, Nanjing 211500, China
7Shihezi University, Shihezi 832003, China
8Blood Transfusion Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China
9Breast Surgery Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China

Correspondence should be addressed to Tianhua Yu; moc.621@mm_htuy and Changlong Song; moc.621@a51lgnahcgnoS

Received 1 October 2017; Accepted 4 December 2017; Published 7 February 2018

Academic Editor: Majeti N. V. R. Kumar

Copyright © 2018 Lirong Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Citrus aurantium is rich in flavonoids, which may prevent osteosarcoma progression, but its related molecular mechanism remains unclear. Flavonoids were extracted from C. aurantium and purified by reparative HPLC. Each fraction was identified by using electrospray ionisation mass spectrometry (ESI-MS). Three main components (naringin, naringenin, and hesperetin) were isolated from C. aurantium. Naringenin inhibited the growth of MG-63 cells, whereas naringin and hesperetin had no inhibitory function on cell growth. ROS production was increased in naringin- and hesperetin-treated groups after one day of culture while the level was always lowest in the naringenin-treated group after three days of culture. 95 osteosarcoma patients who underwent surgery were assigned into two groups: naringenin group (NG, received 20 mg naringenin daily, ) and control group (CG, received 20 mg placebo daily, ). After an average of two-year follow-up, osteosarcoma volumes were smaller in the NG group than in the CG group (). The rate of osteosarcoma recurrence was also lower in the NG group than in CG group. ROS levels were lower in the NG group than in the CG group. Thus, naringenin from Citrus aurantium inhibits osteosarcoma progression and local recurrence in the patients who underwent osteosarcoma surgery by improving antioxidant capability.