Upregulation of Heme Oxygenase-1 by Hemin Alleviates Sepsis-Induced Muscle Wasting in Mice

Figure 3

Hemin induces high HO-1 expression and increased HO-1 activation. (a) Hemin pretreatment can promote HO-1 mRNA expression at 1, 4, and 7 days after surgery ( vs. CLP at 1 day, vs. CLP at 4 days, vs. CLP at 7 days). (b) Hemin enhanced the enzyme activity of HO-1, but ZnPP treatment reversed this effect ( vs. CLP, vs. CLP + hemin; ). (c) Western blot analysis of levels of the HO-1 protein. (d) Results of the corresponding semiquantitative analysis of levels of the HO-1 protein based on the optical density measured using the ImageJ software; the data are presented as means ± SEM and are representative of three separate experiments ( vs. control, vs. CLP).

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