Combination of Coenzyme Q₁₀ Intake and Moderate Physical Activity Counteracts Mitochondrial Dysfunctions in a SAMP8 Mouse Model

Table 2

Summary of used antibodies.

Primary antibodies
Antigen	Antibody	Dilution	Brand
PGC-1α	Mouse monoclonal anti-PGC-1α	1 : 1000	Millipore
TFAM	Rabbit monoclonal anti-TFAM	1 : 2000	Abcam
VDAC1	Mouse monoclonal anti-VDAC1	1 : 1000	Abcam
H2B	Rabbit polyclonal anti-H2B	1 : 1000	Abcam
β-Actin	Mouse monoclonal anti-β-actin	1 : 1000	Cell Signaling Technology
Caspase 3	Rabbit polyclonal anti-caspase 3	1 : 1000	Cell Signaling Technology
Cleaved caspase 3	Rabbit polyclonal anti-cleaved caspase 3 (Asp175)	1 : 1000	Cell Signaling Technology
SIRT5	Rabbit monoclonal anti-SIRT5	1 : 1000	Cell Signaling Technology
Secondary antibodies
Antibody		Dilution	Brand
HRP-conjugated goat anti-mouse IgG (H&L)		1 : 3000	Calbiochem
HRP-conjugated goat anti-rabbit IgG (H&L)		1 : 20000	Sigma-Aldrich