Research Article
Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells
Figure 1
Melatonin protects RPE cells from H2O2 damage. ARPE-19 cells were treated with different concentrations of melatonin, luzindole (melatonin receptor antagonist), and/or H2O2. MTT assay was preformed to measure the viability of ARPE-19 cells after melatonin exposed for 48 h (a). MTT assay was preformed to measure the viability of ARPE-19 cells after H2O2 exposed for 24 h (b). MTT assay was preformed to measure the viability of ARPE-19 cells which pretreatment melatonin for 48 h and then H2O2 exposed for 24 h (c). Cell cytotoxicity was determined using the LDH release assay (d). MTT assay was preformed to measure the viability of ARPE-19 cells after luzindole exposed for 1 h (e). Lipid peroxidation was measured using the TBARS assay (f). Values are the mean ± SD. versus the control group, comparison between cells treated with and without luzindole, and versus H2O2-treated cells.
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