Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells

<div>Melatonin inhibits H<sub>2</sub>O<sub>2</sub>-induced ROS production and apoptosis in RPE cells. RPE cells were pretreated with different concentrations of melatonin (50 and 100 <i>μ</i>M) for 48 h and exposed to 300 <i>μ</i>M H<sub>2</sub>O<sub>2</sub> for 4 h/24 h. ROS production was determined using the DCFDA assay (a, b). Cell apoptosis was analyzed with PI and annexin V (c, d). 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells 