Comparative Analysis of the Effects of Olive Oil Hydroxytyrosol and Its 5-S-Lipoyl Conjugate in Protecting Human Erythrocytes from Mercury Toxicity

Figure 5

Analysis of the reaction mixture of Lipo-HT with Hg²⁺ ions at pH 7.4. (a) Total ion current (TIC) chromatograms of the reaction mixture of Lipo-HT (50 μM) with Hg²⁺ (100 μM) at pH 7.4 (top: before addition of Hg²⁺, bottom: 5 min after addition of Hg²⁺). (b) Top: MS spectrum of the product eluted at 25 min; bottom: inset showing the Hg isotopic signatures of the complex. (c) MS spectrum of the product eluted at 22 min.

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