Research Article

Andrographolide Ameliorates Diabetic Cardiomyopathy in Mice by Blockage of Oxidative Damage and NF-κB-Mediated Inflammation

Figure 9

Andro mitigates HG-induced apoptosis in H9c2 cells. (a) TUNEL staining in H9c2 cells (scale bar = 20 μm). (b) Quantitative analysis of the proportion of TUNEL-positive nuclei. (c) Western blot analysis of Bax, Bcl-2, and caspase-3 activity in H9c2 cells. (d) Bax/Bcl-2 ratio in H9c2 cells. (e) Flow cytometry to determine apoptosis in H9c2 cells. (f) Quantitative analysis of apoptotic cells. NG, 5.5 mM glucose; HG, 25 mM glucose. compared to NG; compared to HG.