Effects of the Aphanizomenon flos-aquae Extract (Klamin®) on a Neurodegeneration Cellular Model

Figure 3

AFA extract protects LAN5 cells from oxidative insult. (a) Viability assay on untreated cells (CTR) or cells treated with TBH or cotreated with TBH and AFA extracts at increasing concentrations for 3 hours. (b) Representative morphological images of untreated cells (CTR) or cells treated with TBH or cotreated with TBH and the AFA extract and treated with the AFA extract. (c) Oxidation kinetics of LAN5 cells alone (CTR) or in the presence of TBH or TBH and AFA at two concentrations measured by the DCFH-DA assay. (d) Fluorescence microscopy images of untreated cells (CTR) and cells treated with TBH or cotreated with TBH and the AFA extract. Bar: 50 μm. AFA extract protects against mitochondrial damage. (e) Fluorescence microscope images of cells untreated (CTR) or treated with TBH alone or with the AFA extract or with CCCP and submitted to JC-1. (f) Values of the ratio between the red and green fluorescence intensities as compared with the control. (g) MitoSOX assay on control cells (CTR) and cells treated with TBH, with TBH and the AFA extract, with AFA alone, and with CCCP. (h) Histogram representing the red fluorescence intensity. Bar: 50 μm.

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