miR-200a Modulates the Expression of the DNA Repair Protein OGG1 Playing a Role in Aging of Primary Human Keratinocytes

Figure 4

OGG1 isoform expression and inflammation in aged primary keratinocytes. (a) OGG1-1a and OGG1-2a expressions were evaluated by quantitative RT-PCR analysis on RNA extracted from young and old donors at 2nd passage. Data were shown as fold change (, , by Student’s test). (b) NLRP3, caspase 1, and IL-1β expressions were evaluated by quantitative RT-PCR analysis on RNA extracted from young and old donor cultures at 2nd passage. Data were shown as fold change (, by Student’s test). (c) Caspase 1 expression was evaluated by Western blot analysis on cell extract from young and old donor cultures at 2nd passage. Both pro-caspase 1 and cleaved caspase 1 forms were indicated. Expression levels of pro-caspase 1 and GAPDH proteins were evaluated by densitometric analysis and normalized by tubulin levels. Data were shown as fold change (, by Student’s test). (d) IL-1β secretion was assayed by antibody arrays using supernatants collected from young and old donor cultures at 2nd passage. Signal intensity (SI) was obtained by densitometric analysis and normalized with cell number (, by Student’s test).

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