Resveratrol Decreases Oxidative Stress by Restoring Mitophagy and Improves the Pathophysiology of Dystrophin-Deficient mdx Mice

Figure 4

Resveratrol induces mitophagy in C2C12 cells. (a) Representative images of EGFP-LC3 and MitoTracker Red (MTR) and the merged images in C2C12 cells. Cells were cultured in the absence or presence of resveratrol (RSV, 30 μM), chloroquine (CQ, 50 μM), or RSV and CQ together, for 6 h. Yellow indicates EGFP-LC3 dots (green) colocalized with mitochondria (red). Scale bar: 10 μm. (b) Summary data of the number of LC3 dots per cell. (c) Summary data of the number of EGFP-LC3 dots colocalized with fragmented mitochondria per cell. In (c) and (d), the data were obtained from 40 randomly selected cells from 4 independent experiments. ; .

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